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KMID : 0380020030180060466
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Korean Journal of Biotechnology and Bioengineering
2003 Volume.18 No. 6 p.466 ~ p.472
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Isolation and Characterization of Microorganisms for the Development of Fermentation Accelerator of Animal Manure
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Kim So-Young
Kim Hong
Chae Hee-Jung
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Abstract
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Several microorganisms were isolated and characterized for the development of fermentation accelerator of animal manure. Firstly, 61 species were isolated from rice bran extract. Secondly, five strains of microorganisms were screened by the analysis of hydrolysis activities for organic compounds including protease, cellulase, amylase, and lipase. From a deodorization test for ammonia gas using the isolated strains, finally three bacterial strains were selected (NA 2, 12, 15). The selected strains, NA 2 and 15 were identified as Bacillus acidocaldarius and Planococcus sp. respectively. The media composition of key nutrients and pH for the mixed culture of the three selected strains were optimized using an experimental design method (response surface method) as follows : beef extract (4.59g/L), peptone (8.72g/L) and pH 6.3. Consequently, the isolated microorganisms seem to have potential applicability in the animal manure treatment.
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KEYWORD
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Microorganisms, fermentation accelerator, animal manure
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